Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: Ion extraction chromatograms (XICs) recorded in MRM mode in a calibration sample analysis. XICs (intensity vs time) for RBV, TVR, BOC, DAC, SIM, SOF and SOFM. Blue and red traces are relative to the quantifier and the qualifier transitions, respectively (see Table 1). Retention times are signaled by a maximum in intensity in both of them. The quantifier/qualifier area ratio (±10%) at analyte retention time is a typical qualifying parameter for each analyte.

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques: Extraction

Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: Superimposition of XICs recorded in MRM mode in four DPS samples analysis. Superimposed XICs traces (intensity vs time) recorded in case of DPS analyses of samples from patients receiving different combined antiviral therapies. A. RBV (blue and red traces, tr = 0.8 min), IS (green and grey traces, tr = 0.8 min), SOFM (light blue and pink traces, tr = 1.3 min) and SOF (dark green and purple, tr = 2.3 min). B. RBV (blue and red traces, tr = 0.8 min), IS (green and grey traces, tr = 0.8 min), DAC (light blue and pink traces, tr = 2.3 min) and SIM (dark green and purple, tr = 3.1 min). C. RBV (blue and red traces, tr = 0.8 min), IS (green and grey traces, tr = 0.8 min), and BOC (light blue and pink traces, tr = 2.5 min). D. RBV (blue and red traces, tr = 0.8 min), IS (green and grey traces, tr = 0.8 min), and TVR (light blue and pink traces, tr = 2.7 min).

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques:

Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: Extraction efficiency of three different sample preparation methods. Under the same instrumental settings, we recorded the peak area of analytes spiked in blank plasma sample extracts (A), and in spiked samples (B). Extractions were performed and analyzed in triplicate (n = 3) with each method. The mean extraction procedure recovery (mean exRE) was calculated as the ratio of the mean peak area of the analyte spiked before extraction to the mean peak area of the analytes spiked after extraction (B/A) multiplied by 100.

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques: Extraction, Sample Prep, Clinical Proteomics

Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: Matrix effect (ME), recovery (exRE) and method efficiency (EF). ME, exRE, EF were calculated on three concentration levels in triplicate (n = 3) as indicated in the table. We recorded analyte peak areas in: standard solutions (A); spiked extracts of blank plasma (B); and extracts from spiked plasma (C). A2 is the ratio of the peak area of the analyte on the IS in standard solutions (A2 = A an /A IS ). B2 is the ratio of the peak area of the analyte on the IS in spiked extracts of blank plasma (B2 = B an /B IS ). C2 is the ratio of the peak area of the analytes on the IS in extracts from spiked plasma (C2 = C an /C IS ). The matrix effect (ME) is expressed as B/A multiplied by 100. The extraction procedure recovery (exRE) is calculated as C/B multiplied by 100 (cfr. for Method 1). The overall analytical recovery (anRE) calculated as C2/B2 multiplied by 100.

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques: Concentration Assay, Clinical Proteomics, Extraction

Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: Intra- and inter-day precision and accuracy of the assay for liquid plasma and DPS. Precision was expressed by the CV%, accuracy by the BIAS% relatively to nominal values assigned for each of the three control levels for every drug analyzed both intra- (n = 10) and inter-day (n = 10). In all cases precision was better than ± 10% and accuracy was within ± 15% of the BIAS% range.

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques: Clinical Proteomics, Control

Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: Post-column infusion experiment. Superimposition of the quantifier transition XICs (Y% axis vs time) obtained in post-column infusion conditions (red traces) to typical XICs (blue traces) for RBV, TVR, DAC, SOF, BOC, SIM and SOFM.

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques:

Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: Liquid plasma vs DPS sample method comparison. Passing-Bablok regression of results obtained on 30 liquid plasma and corresponding DPS samples for RBV, TVR, DAC, SOF, BOC, SIM and SOFM, as indicated. Parameters of the linear correlation, reported in each box, indicate stringent correlation of data obtained with the two methods for all drugs.

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques: Clinical Proteomics, Comparison

Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: On-bench stability of liquid plasma samples and their extracts. (A.) Mean results obtained in the analysis of centrifuged/acidified plasma quality control samples (at indicated low, medium and high concentration values) kept on-bench at RT for 0, 4, 8, 24 h are reported. (B.) Mean results obtained for extracts of the same samples kept on bench at the same time of respective plasmas. Analyses were performed in triplicate for each sample and time point.

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques: Clinical Proteomics, Control, Concentration Assay

Journal: Clinical Mass Spectrometry

Article Title: Multiplexed therapeutic drug monitoring (TDM) of antiviral drugs by LC–MS/MS

doi: 10.1016/j.clinms.2017.12.002

Figure Lengend Snippet: Time-course of the drug levels (measured both in liquid plasma and DPS samples) in a patient under therapy. Time course of drug trough concentrations in plasma samples from four different patients receiving different combined INF-free antiviral therapies. Concentration of the drugs (ng/ml) are plotted vs time (days) since the beginning of therapy. TDM was performed every other week for three months. A. Blue diamond = liquid plasma RBV, purple cross = DPS RBV, red square = liquid plasma SOF, light blue star = DPS SOF, green triangle = liquid plasma SOFM and orange circle = DPS SOFM concentration respectively. B. Blue diamond = liquid plasma RBV, red square = DPS RBV, green triangle = liquid plasma DAC, purple cross = DPS DAC, light blue star = liquid plasma SIM and orange circle = DPS SIM concentration respectively. C. Blue diamond = liquid plasma RBV, red square = DPS RBV, green triangle = liquid plasma BOC, purple cross = DPS BOC concentration respectively. D. Blue diamond = liquid plasma RBV, red square = DPS RBV, green triangle = liquid plasma TVR, purple cross = DPS TVR concentration respectively.

Article Snippet: RBV, TVR, BOC, DAC, SIM, SOF, SOFM and [ 13 C 5 ]RBV, as an internal standard (IS) were purchased from Alsachim (Strasbourg, France).

Techniques: Clinical Proteomics, Concentration Assay